ASTRA® C18-Hybrid column

Estrogens Analysis with ASTRA® C18-Hybrid column

ABSTRACT:

This application describes a quick and robust LC-MS/MS method for analysis of estrone (E1 – shown in the upper

chromatograms) and estradiol (E2 – shown in the lower chromatograms), two naturally occurring estrogens in

the human body. The ASTRA® C18-Hybrid column provides perfect selectivity and peak symmetry for target

compounds throughout a long injection sequence.

INTRODUCTION:

Analysis of endogenous Estrogens is challenging due to the low physiological concentrations and weak UV

absorption. Multiple metabolites with similar chemical structures and close molecular weights as well as

co-eluting substances from biological fluids may cause significant ion suppression in mass spectrometry.

Ionization of estrogens is inefficient, introduction of derivatization agents may enhance ionization, but

non-specific product ions can be generated. Therefore, the method generating derivatives with product ions

specific to individual Estrogens is highly demanded.